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human cd80 pe conjugated antibody  (R&D Systems)


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    R&D Systems human cd80 pe conjugated antibody
    Human Cd80 Pe Conjugated Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+b7+1/Human+B7-1%2FCD80+PE-conjugated+Antibody/pm41564474-131-13-18
    Average 94 stars, based on 6 article reviews
    human cd80 pe conjugated antibody - by Bioz Stars, 2026-10
    94/100 stars

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    Selection:

    Article Title: CTLA-4 protein variants
    Article Snippet: .. Selection for Improved Potency Ribosome display affinity based selections were performed using reducing concentrations of human B7-1 (RnD Systems, 140-B1) and/or B7-2 (RnD Systems, 141-B2) as described in Hanes et al. (supra). .. In brief, for each round of selection the library was incubated with the B7 molecules, the Fc fused B7 was coupled to protein G coated paramagnetic beads (Dynal M280)® and bound tertiary complexes (mRNA-ribosome-scFv) were recovered by magnetic separation whilst unbound complexes were washed away.

    Recombinant:

    Article Title: Nonimmune cell–derived ICOS ligand functions as a renoprotective αvβ3 integrin–selective antagonist
    Article Snippet: .. Recombinant proteins used in this study were as follows: human integrin αvβ3 (R&D Systems, 3050-AV), human integrin α3β1 (R&D Systems, 2840-A3), human integrin αIIbβ3 (R&D Systems, 7148-A2), human integrin αvβ5 (R&D Systems, 2528-AV), human fibronectin (R&D Systems, 4305-FNB-200), human ICOSL-His Tag (Thermo Fisher Scientific, 11559H08H50), mouse integrin αvβ3 (R&D Systems, 7889-AV-050), mouse ICOSL (Sino Biological, 50-190-M08H25), human B7.1 (R&D Systems, 140-B1), and human ICOS (R&D Systems, 169-CS). .. Antibodies used were as follows: mouse anti-human αvβ3 integrin (LM609 clone; Millipore, MAB1976), rabbit anti-His tag (Rockland, 600-401-382), rabbit IgG (Gene Tex, GTX35035), mouse anti-human synaptopodin (D-9; Santa Cruz Biotechnology, sc-515842), rabbit anti-ICOSL (MyBioSource, MBS6004943), and custom polyclonal rabbit anti-ICOSL (GenScript; www.genscript.com ; a peptide corresponding to amino acids YPRPNVYWINKTDNC was conjugated to KLH via an N-terminal cysteine residue and the final serum titer was >1:100,000 by ELISA).

    Article Title: Feline immunodeficiency virus infection is characterized by B7+CTLA4+ T cell apoptosis.
    Article Snippet: Cell lysates and supernatant were harvested and frozen at 270 C. Infection-transfection stocks were plaque-purified as described elsewhere [47], with modification, by use of the presence of E. coli b-glucuronidase and substituting the substrate 5-bromo-6-chloro-3-indolyl-b-d-galactopyranoside (BIOSYNTH). .. Confirmation of expression of feline B7.1 and feline B7.2 from recombinant viruses, SPV/B7.1-HIS and SPV/B7.2-HIS, respectively, was done by standard Western blot methods [48] with use of polyclonal goat anti–human B7.1 and goat anti–human B7.2 antibodies (R&D Systems). ..

    Article Title: ICOSL for use as a renal therapeutic
    Article Snippet: .. Recombinant proteins used in this study were as follows: human integrin αvβ3 (R&D Systems, 3050-AV), human integrin α3β1 (R&D Systems, 2840-A3), human integrin αIIbβ3 (R&D Systems, 7148-A2), human integrin αvβ5 (R&D Systems, 2528-AV), human fibronectin (R&D Systems, 4305-FNB-200), human ICOSL-His Tag (Thermo Fisher Scientific, 11559H08H50), mouse integrin αvβ3 (R&D Systems, 7889-AV-050), mouse ICOSL (Sino Biological, 50-190-M08H25), human B7.1 (R&D Systems, 140-B1), and human ICOS (R&D Systems, 169-CS). .. Antibodies used were as follows: mouse anti-human αvβ3 integrin (LM609 clone; Millipore, MAB1976), rabbit anti-His tag (Rockland, 600-401-382), rabbit IgG (Gene Tex, GTX35035), mouse anti-human synaptopodin (D-9; Santa Cruz Biotechnology, sc-515842), rabbit anti-ICOSL (MyBioSource, MBS6004943), and custom polyclonal rabbit anti-ICOSL (GenScript; www.genscript.com; a peptide corresponding to amino acids YPRPNVYWINKTDNC (SEQ ID NO: 3) was conjugated to KLH via an N-terminal cysteine residue and the final serum titer was >1:100,000 by ELISA).

    Expressing:

    Article Title: Feline immunodeficiency virus infection is characterized by B7+CTLA4+ T cell apoptosis.
    Article Snippet: Cell lysates and supernatant were harvested and frozen at 270 C. Infection-transfection stocks were plaque-purified as described elsewhere [47], with modification, by use of the presence of E. coli b-glucuronidase and substituting the substrate 5-bromo-6-chloro-3-indolyl-b-d-galactopyranoside (BIOSYNTH). .. Confirmation of expression of feline B7.1 and feline B7.2 from recombinant viruses, SPV/B7.1-HIS and SPV/B7.2-HIS, respectively, was done by standard Western blot methods [48] with use of polyclonal goat anti–human B7.1 and goat anti–human B7.2 antibodies (R&D Systems). ..

    Western Blot:

    Article Title: Feline immunodeficiency virus infection is characterized by B7+CTLA4+ T cell apoptosis.
    Article Snippet: Cell lysates and supernatant were harvested and frozen at 270 C. Infection-transfection stocks were plaque-purified as described elsewhere [47], with modification, by use of the presence of E. coli b-glucuronidase and substituting the substrate 5-bromo-6-chloro-3-indolyl-b-d-galactopyranoside (BIOSYNTH). .. Confirmation of expression of feline B7.1 and feline B7.2 from recombinant viruses, SPV/B7.1-HIS and SPV/B7.2-HIS, respectively, was done by standard Western blot methods [48] with use of polyclonal goat anti–human B7.1 and goat anti–human B7.2 antibodies (R&D Systems). ..

    Blocking Assay:

    Article Title: Costimulatory Pathways in Lymphocyte Proliferation Induced by the Simian Immunodeficiency Virus SIVsmmPBj14
    Article Snippet: .. Individual blocking monoclonal antibodies specific for the human B7-1 and B7-2 molecules (R&D Systems) were also used in these experiments to define better the costimulatory pathways at work in this cell system. ..

    Bioprocessing:

    Article Title: Costimulatory Pathways in Lymphocyte Proliferation Induced by the Simian Immunodeficiency Virus SIVsmmPBj14
    Article Snippet: .. Individual blocking monoclonal antibodies specific for the human B7-1 and B7-2 molecules (R&D Systems) were also used in these experiments to define better the costimulatory pathways at work in this cell system. ..



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    Low-affinity CD5 CAR-T cells exhibit reduced fratricide and exhaustion phenotypes (A) Viability of CAR-T cells measured on day 3 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (B) Culture supernatants were collected on day 3 after cell seeding. IFN-γ and TNF-α levels secreted by CAR -T cells were measured by cytometric bead array. Data are presented as mean ± SD from multiple donors ( n = 3). (C) CD69 expression level of CAR-T cells on day 10 after seeding. Fluorescence intensity was quantified by flow cytometry. Data are presented as mean ± SD from multiple donors ( n = 3). (D) Total T cell number harvested on day 10 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (E) Expression levels of PD-1 and LAG-3 (surface markers), and TOX (intracellular marker) in CAR-T cells on day 10 after seeding. Surface markers were analyzed by flow cytometry, and TOX expression was assessed via intracellular staining following fixation and permeabilization. Data are presented as mean ± SD from multiple donors ( n = 3). All statistical significance was assessed using a linear mixed-effects model with donor as a random effect and Tukey’s test.

    Journal: Molecular Therapy Oncology

    Article Title: Antigen-binding affinity is a key determinant of the durable antitumor activity of CD5 CAR-T cells

    doi: 10.1016/j.omton.2026.201158

    Figure Lengend Snippet: Low-affinity CD5 CAR-T cells exhibit reduced fratricide and exhaustion phenotypes (A) Viability of CAR-T cells measured on day 3 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (B) Culture supernatants were collected on day 3 after cell seeding. IFN-γ and TNF-α levels secreted by CAR -T cells were measured by cytometric bead array. Data are presented as mean ± SD from multiple donors ( n = 3). (C) CD69 expression level of CAR-T cells on day 10 after seeding. Fluorescence intensity was quantified by flow cytometry. Data are presented as mean ± SD from multiple donors ( n = 3). (D) Total T cell number harvested on day 10 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (E) Expression levels of PD-1 and LAG-3 (surface markers), and TOX (intracellular marker) in CAR-T cells on day 10 after seeding. Surface markers were analyzed by flow cytometry, and TOX expression was assessed via intracellular staining following fixation and permeabilization. Data are presented as mean ± SD from multiple donors ( n = 3). All statistical significance was assessed using a linear mixed-effects model with donor as a random effect and Tukey’s test.

    Article Snippet: The following antibodies were used: BioLegend, CD5-PE (clone UCHT2), CD69-PE (clone FN50), LAG3-FITC (Clone 7H2C65), mIgG2a-AF647 (clone RMG2a-62), and streptavidin-AF647; BD Biosciences, CD4-V500 (clone RPA-T4), CD8-V450 (clone RPA-T8), αβ TCR-PE (clone IP26), and γδ TCR-BV421 (clone 11F2); Thermo Fisher Scientific, PD-1-PE (clone J105) and CD27-PE (clone O323); Miltenyi Biotec, TOX-APC (clone REA473), Vδ1-PE-Cy7 (clone REA173), and Vδ2-V500 (clone 123R3); Cell Signaling Technology, Myc-AF647 or PE (clone 9B11).

    Techniques: Expressing, Fluorescence, Flow Cytometry, Marker, Staining

    Affinity-tuned A2 variants provide functional evidence that antigen-binding affinity plays a key role in regulating fratricide and T cell exhaustion (A) Binding level of recombinant anti-CD5 scFvs to CD5-positive and CD5-negative cell lines, as measured by flow cytometry. Data are presented as mean ± SD from technical replicates ( n = 3). (B) Affinity properties of anti-CD5 scFvs as determined by biolayer interferometry using Octet system. A2 scFv was used as controls. (For definitions of K D , K a , K dis , and curve fit parameters, refer to legend.) (C) Viability of CAR-T cells measured on day 3 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (D) Culture supernatants were collected on day 3 to assess IFN-γ and TNF-α secretion, measured using cytometric bead array. Data are presented as mean ± SD from technical replicates ( n = 3). (E) Total T cell number harvested on day 10 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (F) CD69 expression levels in CAR-T cells on day 10 after cell seeding, measured by surface staining and flow cytometry. Data are presented as mean ± SD from multiple donors ( n = 3). (G) Expression levels of PD-1 and LAG-3 (surface markers) and TOX (intracellular) in CAR-T cells on day 10 after seeding. Surface markers were analyzed by flow cytometry, and TOX expression was assessed via intracellular staining. Data are presented as mean ± SD from multiple donors ( n = 3). (H) 3 × 10 5 Myc + CAR-T cells were co-cultured with 1 × 10 5 Jurkat cells every 3–4 days for repeated antigen stimulation. Expansion of Myc + CAR-T cells was measured weekly by flow cytometry. Data are presented as mean ± SD from technical replicates ( n = 3). Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparisons test for (A, D, and H) and a linear mixed-effects model with donor as a random effect followed by Tukey’s test for (C–G).

    Journal: Molecular Therapy Oncology

    Article Title: Antigen-binding affinity is a key determinant of the durable antitumor activity of CD5 CAR-T cells

    doi: 10.1016/j.omton.2026.201158

    Figure Lengend Snippet: Affinity-tuned A2 variants provide functional evidence that antigen-binding affinity plays a key role in regulating fratricide and T cell exhaustion (A) Binding level of recombinant anti-CD5 scFvs to CD5-positive and CD5-negative cell lines, as measured by flow cytometry. Data are presented as mean ± SD from technical replicates ( n = 3). (B) Affinity properties of anti-CD5 scFvs as determined by biolayer interferometry using Octet system. A2 scFv was used as controls. (For definitions of K D , K a , K dis , and curve fit parameters, refer to legend.) (C) Viability of CAR-T cells measured on day 3 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (D) Culture supernatants were collected on day 3 to assess IFN-γ and TNF-α secretion, measured using cytometric bead array. Data are presented as mean ± SD from technical replicates ( n = 3). (E) Total T cell number harvested on day 10 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (F) CD69 expression levels in CAR-T cells on day 10 after cell seeding, measured by surface staining and flow cytometry. Data are presented as mean ± SD from multiple donors ( n = 3). (G) Expression levels of PD-1 and LAG-3 (surface markers) and TOX (intracellular) in CAR-T cells on day 10 after seeding. Surface markers were analyzed by flow cytometry, and TOX expression was assessed via intracellular staining. Data are presented as mean ± SD from multiple donors ( n = 3). (H) 3 × 10 5 Myc + CAR-T cells were co-cultured with 1 × 10 5 Jurkat cells every 3–4 days for repeated antigen stimulation. Expansion of Myc + CAR-T cells was measured weekly by flow cytometry. Data are presented as mean ± SD from technical replicates ( n = 3). Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparisons test for (A, D, and H) and a linear mixed-effects model with donor as a random effect followed by Tukey’s test for (C–G).

    Article Snippet: The following antibodies were used: BioLegend, CD5-PE (clone UCHT2), CD69-PE (clone FN50), LAG3-FITC (Clone 7H2C65), mIgG2a-AF647 (clone RMG2a-62), and streptavidin-AF647; BD Biosciences, CD4-V500 (clone RPA-T4), CD8-V450 (clone RPA-T8), αβ TCR-PE (clone IP26), and γδ TCR-BV421 (clone 11F2); Thermo Fisher Scientific, PD-1-PE (clone J105) and CD27-PE (clone O323); Miltenyi Biotec, TOX-APC (clone REA473), Vδ1-PE-Cy7 (clone REA173), and Vδ2-V500 (clone 123R3); Cell Signaling Technology, Myc-AF647 or PE (clone 9B11).

    Techniques: Functional Assay, Binding Assay, Recombinant, Flow Cytometry, Expressing, Staining, Cell Culture

    High-affinity C7 variant-based CAR5 exhibited increased levels of both fratricide and T cell exhaustion compared to the C7 wild-type CAR5 (A) Binding level recombinant anti-CD5 scFvs to CD5-positive and CD5-negative cell lines, as measured by flow cytometry. Data are presented as pooled mean ± SD from technical replicates ( n = 3). (B) Affinity properties of anti-CD5 scFvs as determined by biolayer interferometry using Octet system. C7 scFv was used as controls. (For definitions of K D , K a , K dis , and curve fit parameters, refer to legend.) (C) Viability of CAR-T cells measured on day 3 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (D) Culture supernatants were collected on day 3 to assess IFN-γ and TNF-α secretion, measured using cytometric bead array. Data are presented as mean ± SD from technical replicates ( n = 3). (E) Total T cell number harvested on day 10 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (F) CD69 expression levels in CAR-T cells on day 10 after cell seeding, measured by surface staining and flow cytometry. Data are presented as mean ± SD from multiple donors ( n = 3). (G) Expression levels of PD-1 and LAG-3 (surface markers) and TOX (intracellular) in CAR-T cells on day 10 after seeding. Surface markers were analyzed by flow cytometry, and TOX expression was assessed via intracellular staining. Data are presented as mean ± SD from multiple donors ( n = 3). (H) 3 × 10 5 Myc + CAR-T cells were co-cultured with 1 × 10 5 Jurkat cells every 3–4 days for repeated antigen stimulation. Expansion of Myc + CAR-T cells was measured weekly by flow cytometry. Data are presented as mean ± SD from technical replicates ( n = 3). Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparisons test for (A, D, and H) and a linear mixed-effects model with donor as a random effect followed by Tukey’s test for (C–G).

    Journal: Molecular Therapy Oncology

    Article Title: Antigen-binding affinity is a key determinant of the durable antitumor activity of CD5 CAR-T cells

    doi: 10.1016/j.omton.2026.201158

    Figure Lengend Snippet: High-affinity C7 variant-based CAR5 exhibited increased levels of both fratricide and T cell exhaustion compared to the C7 wild-type CAR5 (A) Binding level recombinant anti-CD5 scFvs to CD5-positive and CD5-negative cell lines, as measured by flow cytometry. Data are presented as pooled mean ± SD from technical replicates ( n = 3). (B) Affinity properties of anti-CD5 scFvs as determined by biolayer interferometry using Octet system. C7 scFv was used as controls. (For definitions of K D , K a , K dis , and curve fit parameters, refer to legend.) (C) Viability of CAR-T cells measured on day 3 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (D) Culture supernatants were collected on day 3 to assess IFN-γ and TNF-α secretion, measured using cytometric bead array. Data are presented as mean ± SD from technical replicates ( n = 3). (E) Total T cell number harvested on day 10 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (F) CD69 expression levels in CAR-T cells on day 10 after cell seeding, measured by surface staining and flow cytometry. Data are presented as mean ± SD from multiple donors ( n = 3). (G) Expression levels of PD-1 and LAG-3 (surface markers) and TOX (intracellular) in CAR-T cells on day 10 after seeding. Surface markers were analyzed by flow cytometry, and TOX expression was assessed via intracellular staining. Data are presented as mean ± SD from multiple donors ( n = 3). (H) 3 × 10 5 Myc + CAR-T cells were co-cultured with 1 × 10 5 Jurkat cells every 3–4 days for repeated antigen stimulation. Expansion of Myc + CAR-T cells was measured weekly by flow cytometry. Data are presented as mean ± SD from technical replicates ( n = 3). Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparisons test for (A, D, and H) and a linear mixed-effects model with donor as a random effect followed by Tukey’s test for (C–G).

    Article Snippet: The following antibodies were used: BioLegend, CD5-PE (clone UCHT2), CD69-PE (clone FN50), LAG3-FITC (Clone 7H2C65), mIgG2a-AF647 (clone RMG2a-62), and streptavidin-AF647; BD Biosciences, CD4-V500 (clone RPA-T4), CD8-V450 (clone RPA-T8), αβ TCR-PE (clone IP26), and γδ TCR-BV421 (clone 11F2); Thermo Fisher Scientific, PD-1-PE (clone J105) and CD27-PE (clone O323); Miltenyi Biotec, TOX-APC (clone REA473), Vδ1-PE-Cy7 (clone REA173), and Vδ2-V500 (clone 123R3); Cell Signaling Technology, Myc-AF647 or PE (clone 9B11).

    Techniques: Variant Assay, Binding Assay, Recombinant, Flow Cytometry, Expressing, Staining, Cell Culture